ActoFactor™ Recombinant Mouse Nucleobindin 2

Specification

Cat.No.

CSC-CTK0799

Description

Nucleobindin-2 (NUCB-2) is an EF-hand calcium-binding protein. Nucleobindin-2 takes part in calcium homeostasis and is a multifunctional protein that interacts with Ca(2+) nucleic acids and various regulatory proteins in different signaling pathways. NUCB-2 is localized in neuronal perikarya and dendrites of mouse brain. Recombinant mouse NUCB-2 fused to the N-terminal His-tag produced in E. coli is a single, non- glycosylated polypeptide chain (amino acids 25-420) of 417 amino acids and a molecular mass of 49 kDa. NUCB-2 is fused to a 21 amino acid His-tag at the N-terminus and purified by proprietary chromatographic techniques.

Species

Mouse

Product Overview

Mouse Nucb2 expressed in E.coli

Molecular Mass

49 kDa

Size

CAT# CSC-CTK0799-25 (25 μg); CAT# CSC-CTK0799-125 (125 μg)

Expression System

E.coli

Purity

Greater than 95% as determined by SDS-PAGE analysis.

Activity

N/A

Endotoxin Level

Less than 1 EU/μg.

Formulation

Sterile-filtered, colorless protein solution (1 mg/ ml) containing 20 mM Tris-HCl (pH 8.0), 0.1 M NaCl and 1 mM EDTA.

Reconstitution

Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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