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ActoFactor™ Recombinant Mouse Interleukin 6
- Specification
Cat.No.
CSC-CTK0003
Description
Interleukin 6 is a multifunctional cytokine that plays an important role in host defense, acute phase reactions, immune responses and hematopoiesis. IL-6 is expressed by a variety of cell types including T cells, B cells, monocytes/macrophages, fibroblasts, hepatocytes, vascular endothelial cells and various tumor cell lines. IL-6 exerts biological effects on B cells, T cells, hepatocytes and myeloid hematopoietic progenitors. The methionyl form of recombinant mouse IL-6 contains 188 amino acid residues with a predicted molecular mass of approximately a 21.8 kDa.
Species
Mouse
Product Overview
Mouse Il6 expressed in E.coli
Sequence
A DNA sequence encoding the mature mouse IL-6 protein was expressed in E. coli.
Size
CAT# CSC-CTK0003-5 (5 μg); CAT# CSC-CTK0003-25 (25 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The biological activity of recombinant mouse IL-6 is measured in a cell proliferation assay using a mouse factor-dependent plasmacytoma cell line, T1165. The ED50 for this effect is typically 0.02 - 0.06 ng/mL.
Endotoxin Level
Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
Formulation
Recombinant mouse IL-6 is lyophilized from a 0.2 μm filtered solution in 50 mM sodium acetate, 1 mM EDTA and 5% trehalose, pH 4.0 containing 50 μg bovine serum albumin per 1 μg cytokine.
Reconstitution
Reconstitute mouse IL-6 at a concentration greater than 10 μg/mL with sterile phosphate buffered saline (PBS) containing at least 0.1% human or bovine serum albumin.
Storage & Stability
Lyophilized mouse IL-6 is stable for upto twelve months from date of receipt at -20°C to -70°C. Reconstituted mouseIL-6 can be stored under sterile conditions at
2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer)for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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