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ActoFactor™ Recombinant Mouse Interleukin 4
- Specification
Cat.No.
CSC-CTK0080
Description
Mouse Interleukin 4 (IL-4) is produced by activated Th2 cells, mast cells and basophils. IL-4 is a pleiotropic immune regulatory cytokine that promotes growth of human B cells; induces expression of germ line Cε transcripts, directs na?ve B cells to switch to the synthesis of IgE and IgG4, and induces expression of CD23 and ClassII-MHC. In monocytes, IL-4 down-regulates FcγR expression; inhibits synthesis of proinflammatory cytokines, including TNF-α, IL-1β, IL-6 and IL-8; induces monocyte/macrophage fusion and mannose receptor expression. IL-4 inhibits the synthesis of IL-12, nitric oxide synthase and cyclooxigenase-2. The mouse IL-4 cDNA encodes a 119 amino acid residue protein that has a molecular mass of 14 kDa. Mouse IL-4 is species specific. IL-4 binds to a heterodimeric receptor formed by the binding subunit IL-4Rα and the γ chain of IL-2R.
Species
Mouse
Product Overview
Mouse Il4 expressed in E.coli
Sequence
A DNA sequence encoding the mature mouse IL-4 protein minus the first two amino acid residues was expressed E. coli.
Size
CAT# CSC-CTK0080-10 (10 μg); CAT# CSC-CTK0080-50 (50 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
Activity was determined in a cell proliferation assay using a mouse factor-dependent cell line HT-2 and the ED50 using this assay was typically 1 – 2 ng/mL.
Endotoxin Level
Endotoxin level is less than 0.1 ng per μg of the cytokine, as determined by the LAL method.
Formulation
Lyophilized from a sterile-filtered solution in PBS containing 50 μg of bovine serum albumin per 1 μg of cytokine.
Reconstitution
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 μg /mL of the cytokine.
Storage & Stability
The lyophilized sample is stable for greater than six months at -20°C to -70°C. Reconstituted mouse IL-4 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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