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ActoFactor™ Recombinant Mouse Interleukin 33
- Specification
Cat.No.
CSC-CTK0704
Description
IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ST2 receptor activating NF-kappaB and MAP kinases. IL-33 induces production of TH2 cell related cytokines, including IL-4, IL-5 and IL-13, and exerts multiple inflammation related bioactivities. Recombinant murine IL-33 is a 17.5 kDa protein containing 158 amino acid residues.
Species
Mouse
Product Overview
Mouse Il33 expressed in E.coli
Molecular Mass
17.5 kDa
Size
CAT# CSC-CTK0704-10 (10 μg); CAT# CSC-CTK0704-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
The ED50 as determined by the dose-dependent stimulation of the proliferation of murine D10S cells is ≤ 0.5 ng/ml, corresponding to a specific activity of Greater than 2 x 10^6 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered solution in 10 mM sodium phosphate (pH 7.5) and 75 mM NaCl.
Reconstitution
Please centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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