ActoFactor™ Recombinant Mouse Interleukin 22

Specification

Cat.No.

CSC-CTK0701

Description

Recombinant murine IL-22 produced in E. coli is a non-disulfide-linked homodimeric, non- glycosylated protein containing 2 x 146 amino acid chains and having a total molecular mass of 34,510 Dalton.

Species

Mouse

Product Overview

Mouse Il22 expressed in E.coli

Molecular Mass

34.5 kDa

Size

CAT# CSC-CTK0701-10 (10 μg); CAT# CSC-CTK0701-50 (50 μg)

Expression System

E.coli

Purity

Greater than 97% as determined by SDS-PAGE and RP-HPLC analysis.

Activity

The ED50 as determined by ist ability to induce IL-10 secretion in Colo205 cells is less than 0.5 μg/ml, corresponding to a specific activity of 2.0 x 10^6 IU/mg.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from 1 x PBS.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu IL-22 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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