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ActoFactor™ Recombinant Mouse Interleukin 2
- Specification
Cat.No.
CSC-CTK0019
Description
Interleukin 2 (IL-2) is produced by activated T cells. IL-2 was first described as a T cell growth factor. It is now known to stimulate growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes. Mouse IL-2 is species-
specific and is inactive on human cells. The biological activity of IL-2 is mediated by the binding to a cell surface receptor. The functional high-affinity receptor of IL-2 is composed of three distinct polypeptide chains, the IL-2 receptor a, b and g subunits. The intermediate-affinity IL-2 receptor complex, which lacks the a subunit, but contains both the b and g subunits, is also capable of transducing the IL-2 signal. The g chain of the IL-2 receptor complex has been shown to be a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-15. Biological activity of IL-2 is mediated by JAK1 and JAK3-induced activation of STAT3 and STAT5. Recombinant mouse IL-2, containing a mixture of the 149 amino acid residue protein and the 150 amino acid residue N-methionyl form, has a predicted molecular mass of approximately 17.2 kDa.
Species
Mouse
Product Overview
Mouse Il2 expressed in E.coli
Sequence
A DNA sequence encoding the mature mouse IL-2 protein was expressed in E. coli.
Size
CAT# CSC-CTK0019-20 (20 μg); CAT# CSC-CTK0019-100 (100 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The ED50 of BMP-2 is typically 150 - 600 ng/mL as measured by its ability to induce alkaline phosphatase production by mouse ATDC5 chondrogenic cells.
Endotoxin Level
Endotoxin level less than 0.1 ng per 1 mg of the cytokine as determined by the LAL method.
Formulation
Lyophilized from a sterile filtered solution in 50 mM ammonium acetate and 1 mM DTT, pH 4.0, containing 50 mg of bovine serum albumin per 1 mg of cytokine.
Reconstitution
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/mL.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted mouse IL-2 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C-70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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