Featured Products
- HCC-78
- HDLM-2
- DOHH-2
- L-540
- MX-1
- NALM-6
- NB-4
- CAL-51
- SNB-19
- KYSE-520
- MKN-45
- BA/F3
- MS-5
- HCEC-B4G12
- NK-92
- PA-TU-8988S
- MONO-MAC-1
- PA-TU-8902
- Human Microglia
- Human Hepatic Stellate Cells
- Human Skeletal Muscle Cells (DMD)
- Human Schwann Cells
- Human Oral Keratinocytes (HOK)
- Human Cardiomyocytes
- Human Small Intestinal Epithelial Cells
- Human Colonic Epithelial Cells
- Human Intestinal Fibroblasts
- Primary Human Large Intestine Microvascular Endothelial Cells
- Human Small Intestinal Microvascular Endothelial Cells
- Human Retinal Pigment Epithelial Cells
- Human Hepatocytes
- Cynomolgus Monkey Lung Microvascular Endothelial Cells
- Cynomolgus Monkey Vein Endothelial Cells
- C57BL/6 Mouse Primary Mammary Epithelial Cells
- C57BL/6 Mouse Vein Endothelial Cells
- Rat Primary Kidney Epithelial Cells
- Rat Gingival Epithelial Cells
- Rabbit Lung Endothelial Cells
Our Promise to You
Guaranteed product quality, expert customer support
ActoFactor™ Recombinant Mouse Interleukin 17A
- Specification
Cat.No.
CSC-CTK0694
Description
IL-17 is a potent proinflammatory cytokine produced by activated memory T cells. There are at least six members of the IL-17 family in humans and in mice. As Interleukin-17 shares properties with IL-1 and TNF-alpha, it may induce joint inflammation and bone and cartilage destruction. This cytokine is found in synovial fluids of patients with rheumatoid arthritis, and produced by rheumatoid arthritis synovium. It increases IL-6 production, induces collagen degradation and decreases collagen synthesis by synovium and cartilage and proteoglycan synthesis in cartilage. Interleukin-17 is also able to increase bone destruction and reduce its formation. Blocking of Interleukin-17 with specific inhibitors provides a protective inhibition of cartilage and bone degradation. Recombinant murine IL-17 produced in E. coli is a homodimeric, non-glycosylated polypeptide chain of 266 amino acids and a molecular mass of 29,956 Dalton.
Species
Mouse
Product Overview
Mouse Il17a expressed in E.coli
Molecular Mass
29.96 kDa
Size
CAT# CSC-CTK0694-25 (25 μg); CAT# CSC-CTK0694-125 (125 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
Recombinant mouse IL-17 is fully biologically active when compared to a standard. The ED50 as determined by the dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblast cells was found to be 0.4 ng/ml corresponding to a specific activity of 2.5 x 10^6 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized (freeze-dried), white powder. Lyophiliz ed from a concentrated (1 mg/ml) protein solution containing no additives.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu IL-17 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Ask a Question
Write your own review
ONLINE INQUIRY
