ActoFactor™ Recombinant Mouse Interleukin 15

Specification

Cat.No.

CSC-CTK0692

Description

Recombinant Murine Interleukin-15 (rMu IL-15) is a potent lymphoid cell growth factor which exerts its biological activities primarily on T cells. rMu IL-15 is a 13.3 kDa protein containing 115 amino acid residues.

Species

Mouse

Product Overview

Mouse Il15 expressed in E.coli

Molecular Mass

13.3 kDa

Size

CAT# CSC-CTK0692-10 (10 μg); CAT# CSC-CTK0692-50 (50 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

Activity

Creative Bioarray's murine IL-15 is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells was found to be < 5.0 ng/ml, corresponding to a specific activity of Greater than 2 x 10^5 units/mg.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a sterile-filtered solution in 10 mM Tris (pH 8.0).

Reconstitution

Please centrifuge briefly product before opening the vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.5-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu IL-15 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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