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ActoFactor™ Recombinant Mouse Insulin-like growth factor 1
- Specification
Cat.No.
CSC-CTK0032
Description
Insulin-like growth factor-1 (IGF-1) is structurally homologous to proinsulin. IGF-1 is produced by several cell types and may have autocrine, paracrine and endocrine functions. IGF-1 is a potent mitogen that mediates the growth-promoting activities of growth hormone postnatally. It also plays a role during embryonic growth and differentiation. Recombinant mouse IGF-1 is a 7.6 kDa protein containing 70 amino acid residues. IGF-1 receptor is a disulfide-linked heterotetrameric transmembrane glycoprotein with an intracellular tyrosine kinase domain.
Species
Mouse
Product Overview
Mouse Igf1 expressed in E.coli
Sequence
A DNA sequence encoding the mature IGF-1 protein was expressed in E. coli.
Size
CAT# CSC-CTK0032-50 (50 μg); CAT# CSC-CTK0032-250 (250 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
Activity was measured in a serum-free cell proliferation assay using the human cell line MCF-7 and the ED50 was typically 1.0-3.0 ng/ml.
Endotoxin Level
Endotoxin level is less than 0.1 ng per μg of the cytokine as determined by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 25 μg/mL of the cytokine.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Upon reconstitution, mouse IGF-1 can be stored under sterile conditions at 2° - 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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