ActoFactor™ Recombinant Mouse Fibroblast growth factor 15

Specification

Cat.No.

CSC-CTK0667

Description

FGF-15 takes part in the suppression of bile acid biosynthesis by down-regulation of CYP7A1 expression. FGF-15 is expressed in the developing brain. FGF-15 produced in E. coli is a 23.78 kDa protein containing 203 amino acid residues including a 10 a.a. N-Terminal His-tag.

Species

Mouse

Product Overview

Mouse Fgf15 expressed in E.coli

Molecular Mass

23.8 kDa

Size

CAT# CSC-CTK0667-10 (10 μg); CAT# CSC-CTK0667-50 (50 μg)

Expression System

E.coli

Purity

Greater than 90% as determined by SDS-PAGE analysis.

Activity

N/A

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a filtered (0.4 @m) protein soluti on (0.5 mg/ml) in 20 mM TRIS (pH 8.0) and 20 mM NaCl.

Reconstitution

Please centrifuge product before opening vial. It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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