ActoFactor™ Recombinant Mouse Epidermal growth factor

Specification

Cat.No.

CSC-CTK0661

Description

Epidermal Growth Factor (EGF) is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-alpha and VGF (vaccinia virus growth factor). Recombinant murine EGF is a 6.0 kDa globular protein containing 53 amino acid residues, including 3 intramolecular disulfide-bonds.

Species

Mouse

Product Overview

Mouse Egf expressed in E.coli

Molecular Mass

6.0 kDa

Size

CAT# CSC-CTK0661-500 (500 μg); CAT# CSC-CTK0661-2500 (2.5 mg)

Expression System

E.coli

Purity

Greater than 97% as determined by SDS-PAGE and HPLC analysis.

Activity

The ED50 was determined by a cell proliferation assay using BALB/c 3T3 cells is 1 x 10^7 units/mg.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a sterile-filtered solution in 1XPBS (pH 7.4).

Reconstitution

Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted EGF should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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