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ActoFactor™ Recombinant Mouse Colony stimulating factor 3 (granulocyte)
- Specification
Cat.No.
CSC-CTK0018
Description
Mouse granulocyte colony stimulating factor (G-CSF) is a 19 kDa protein with 179 amino acid residues. G-CSF is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation and activation of hematopoietic cells of the granulocyte lineage.
Species
Mouse
Product Overview
Mouse Csf3 expressed in
Sequence
A DNA sequence encoding the mature mouse G-CSF protein sequence was expressed in E. coli.
Size
CAT# CSC-CTK0018-5 (5 μg); CAT# CSC-CTK0018-25 (25 μg)
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The biological activity of recombinant mouse G-CSF was measured in a cell proliferation assay using a mouse myeloblastic cell line, NSF-60. The ED50 for this effect is typically 0.01 - 0.03 ng/ml, corresponding to a specific activity of approximately 5 x 10^7 units/mg.
Endotoxin Level
Endotoxin level is less than 0.1 ng per mg of mouse G-CSF as determined by the LAL method.
Formulation
Lyophilized from a sterile-filtered solution in 20mM acetic acid containing 50 mg of bovine serum albumin per 1 mg of cytokine.
Reconstitution
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 5 mg/ml of the cytokine.
Storage & Stability
The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted mouse G-CSF can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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