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ActoFactor™ Recombinant Mouse Colony stimulating factor 2 (granulocyte-macrophage)
- Specification
Cat.No.
CSC-CTK0063
Description
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a potent species-specific stimulator of granulocyte-macrophage, eosinophil, megakaryocyte and erythroid progenitors. GM-CSF has also been reported to have a functional role on non-hematopoietic cells including endothelial cells. Additionally, GM-CSF can stimulate the proliferation of a number of tumor-derived cell lines, such as osteogenic sarcoma, carcinoma and adenocarcinoma. GM-CSF binds to a heterodimeric receptor formed by the GM-CSFRα/CD116 binding chain and the signal-transducing common β chain (CD131), which is also a component of the high affinity IL-3 and IL-5 receptors. Recombinant mouse GM-CSF contains 125 amino acids and has a predicted molecular mass of approximately 14.8 kDa.
Species
Mouse
Product Overview
Mouse Csf2 expressed in E.coli
Sequence
A DNA sequence encoding the mature mouse GM-CSF protein (Ala 18 - Lys 141; Accession #CAA26193) was expressed in E. coli.
Size
CAT# CSC-CTK0063-10 (10 μg); CAT# CSC-CTK0063-50 (50 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The biological activity of recombinant mouse GM-CSF is measured by proliferation of the factor-dependent murine cell line, DA-3. The ED50 for this effect is typically 10 - 60 pg/mL.
Endotoxin Level
Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
Formulation
Recombinant mouse GM-CSF is lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (PBS) containing 50 μg bovine serum albumin per 1 μg cytokine.
Reconstitution
Reconstitute mouse GM-CSF at a concentration greater than 10 μg/mL with sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage & Stability
Lyophilized mouse GM-CSF is stable for up totwelve months from date of receipt at -20°C to -70°C. Reconstituted mouse GM-CSF can be stored under sterile conditions at 2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer) for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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