ActoFactor™ Recombinant Mouse Colony stimulating factor 1 (macrophage)

Specification

Cat.No.

CSC-CTK0084

Description

Macrophage-colony stimulating factor (M-CSF or CSF-1) can be produced by a number of cells including fibroblasts, activated macrophages, secretory epithelial cells of the endometrium, bone marrow stromal cells, brain astrocytes, osteoblasts, renal mesangial cells, keratinocytes and LPS- or cytokine-activated endothelial cells. M-CSF stimulates the formation of macrophage colonies from bone marrow hematopoietic progenitor cells. It also induces proliferation of isolated macrophages, enhances antibody-dependent cell-mediated cytotoxicity, primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages and stimulates pinocytosis and osteoclast differentiation. M-CSF is synthesized as a membrane-bound propeptide. After proteolytic processing, M-CSF can be secreted in its soluble form or anchored to the membrane. Both forms are biologically active. Natural M-CSFs are glycosylated, disulfide-linked, homodimeric proteins with molecular weights ranging from 40 - 70 kDa. The carbohydrate moiety is not necessary for biological activity. Several C-terminal variants of the soluble form of M-CSF have been found. The N-terminal of mature M-CSF contains 150 amino acids and is necessary and sufficient for interaction with the M-CSF receptor. The human and mouse forms of the N-terminal are highly conserved (80% homology).

Species

Mouse

Product Overview

Mouse Csf1 expressed in E.coli

Sequence

A DNA sequence encoding the N-terminal 230 amino acid residues of the extracellular domain of the mature mouse M-CSF protein sequence (residues 33 - 262 of the mouse M-CSF precursor) was expressed in E. coli.

Size

CAT# CSC-CTK0084-10 (10 μg); CAT# CSC-CTK0084-50 (50 μg)

Expression System

E.coli

Purity

Greater than 97% as determined by SDS-PAGE and visualized by silver stain

Activity

The biological activity of recombinant mouse M-CSF is measured in a cell proliferation assay using an M-CSF-dependent murine monocytic cell line, M-NFS-60. The ED50 for this effect is typically 0.5 - 1.5 ng/mL.

Endotoxin Level

Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.

Formulation

Recombinant mouse M-CSF is lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (PBS) containing 50 μg bovine serum albumin per 1 μg cytokine.

Reconstitution

Reconstitute mouse M-CSF at a concentration greater than 10 μg/mL with sterile PBS containing at least 0.1% human or bovine serum albumin.

Storage & Stability

Lyophilized mouse M-CSF is stable for upto twelve months from date of receipt at -20°C to -70°C. Reconstituted mouse M-CSF can be stored under sterile conditions at 2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer) for three months without detectable loss of activity. Avoid repeated freezing and thawing.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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