ActoFactor™ Recombinant Mouse Chemokine (C-X-C motif) ligand 12 (alpha)

Specification

Cat.No.

CSC-CTK0778

Description

Recombinant Murine Stromal Cell-Derived Factor-1 alpha (rMu SDF-1 alpha) is a recently discovered protein belonging to the alpha-chemokine (C-X-C) family of cytokines. Creative Bioarrays recombinant murine SDF-1 alpha is a 7.9 kDa protein containing 68 amino acid residues.

Species

Mouse

Product Overview

Mouse Cxcl12 expressed in E.coli

Molecular Mass

7.9 kDa

Size

CAT# CSC-CTK0778-10 (10 μg); CAT# CSC-CTK0778-50 (50 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

Activity

The biological activity was determined by its ability to chemoattract human peripheral blood monocytes. The concentration for this effect was found to be in the range of 50-100 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a sterile-filtered, concentrated p rotein solution in 10 mM acetic acid. The protein may appear as a haze or film which is diffi cult to see at the bottom of the vial.

Reconstitution

Please centrifuge product briefly before opening the vial. The lyophilized protein should be reconstituted sterile, ultrapure in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized SDF-1 alpha, though stable at room temperature for up to 3 months, is best stored desiccated at -20°C. Reconstituted murine SDF-1 alpha should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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