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ActoFactor™ Recombinant Mouse Chemokine (C-C motif) ligand 4
- Specification
Cat.No.
CSC-CTK0748
Description
Recombinant human MIP-1 beta produced in E. coli is a single, non-glycosylated polypeptide chain of 69 amino acids and a molecular mass of 7,808 Dalton.
Species
Mouse
Product Overview
Mouse Ccl4 expressed in E.coli
Molecular Mass
7.8 kDa
Size
CAT# CSC-CTK0748-10 (10 μg); CAT# CSC-CTK0748-50 (50 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Activity
Recombinant MIP-1 is fully biologically active when compared to a standard. The activity is calculated by the ability to chemoattract human blood monocytes using concentrations ranging between 20-100 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1 mg/ml), sterile solution in water containing no add itives.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu MIP-1 beta should be used immediately or stored long- term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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