ActoFactor™ Recombinant Mouse Chemokine (C-C motif) ligand 28

Specification

Cat.No.

CSC-CTK0786

Description

MEC is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. Recombinant murine MEC is a 12.6 kDa protein containing 111 amino acid residues.

Species

Mouse

Product Overview

Mouse Ccl28 expressed in E.coli

Molecular Mass

12.6 kDa

Size

CAT# CSC-CTK0786-20 (20 μg); CAT# CSC-CTK0786-100 (100 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

Activity

Determined by its ability to chemoattract murine lymphocytes using a concentration range of 1- 10 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

The protein was lyophilized from 10 mM NaCitrate, pH 4.0.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu MEC should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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