ActoFactor™ Recombinant Mouse Chemokine (C-C motif) ligand 27A

Specification

Cat.No.

CSC-CTK0740

Description

CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues. Recombinant murine CTACK is a 10.9 kDa protein containing 95 amino acid residues.

Species

Mouse

Product Overview

Mouse Ccl27a expressed in E.coli

Molecular Mass

10.9 kDa

Size

CAT# CSC-CTK0740-20 (20 μg); CAT# CSC-CTK0740-100 (100 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

Activity

Determined by its ability to chemoattract CXCR3 transfected HEK/293 cells using a concentration range of 10.0-100.0 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a sterile-filtered solution with no additives.

Reconstitution

Please centrifuge product briefly before opening the vial. Reconstitute in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted CTACK should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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