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ActoFactor™ Recombinant Mouse CD14 antigen
- Specification
Cat.No.
CSC-CTK0712
Description
CD14 (also known lipopolysaccharide [LPS] receptor) is expressed strongly on monocytes and macrophages and weakly on the surface of neutrophils. CD14 (1-201 a.a.) is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentrations as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation. The myeloid differentiation antigen CD14 acts as the major receptor for bacterial LPS. The dominant form of the recombinant wildtype CD14 is the 50 kDa protein.|The mouse CD14 is produced from mouse CD14 transfected CHO-cells in serum free medium. Before transfection, the complete mouse CD14-cDNA was amplified by PCR and cloned into expression vector p-POL-DHFR. The recombinant mouse CD-14 has been purified by His- tag with Talon (Clontech) and controlled by SDS page.
Species
Mouse
Product Overview
Mouse Cd14 expressed in CHO cells
Molecular Mass
N/A
Size
CAT# CSC-CTK0712-2 2 μg; CAT# CSC-CTK0712-10 (10 μg)
Expression System
CHO cells
Purity
N/A
Activity
N/A
Endotoxin Level
N/A
Formulation
Recombinant mouse CD-14 was lyophilized from a conce ntrated protein solution (1 mg/ml) containing phosphate-buffered saline, pH 7.2.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile-ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Please Note: Always centrifuge product briefly before opening vial. It is recommended to store lyophilized protein long-term at –20°C. Reconstituted protein should be stored at –20°C to – 80°C. When stored at the recommended temperature, this protein is stable for 12 months. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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