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ActoFactor™ Recombinant Mouse Adiponectin (Adipocyte Complement Related Protein of 30 kDa; E. coli)
- Specification
Cat.No.
CSC-CTK0768
Description
Adiponectin (also called Acrp30, AdipoQ) is an adipocyte specific secreted protein that circulates in the plasma. It is induced during adipocyte differentiation and it secretion is stimulated by insulin. Murine adiponectin shares about 83% amino acid identity with that of human. Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.
Species
Mouse
Product Overview
Mouse Adipoq expressed in E.coli
Molecular Mass
N/A
Size
CAT# CSC-CTK0768-25 (25 μg); CAT# CSC-CTK0768-125 (125 μg)
Expression System
E.coli
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Measured by its ability to inhibit proliferation of mouse myeloid cell line M1. The ED50 for the effect is typically 1-7 μg/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a 0.2 μm filtered solution in 25 m M Tris, 0.15 M NaCl (pH 7.4) containing 50 μg of bovine serum albumin (BSA) per 1 μg of adipone ctin.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water or PBS to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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