ActoFactor™ Recombinant Mouse Adiponectin (Adipocyte Complement Related Protein of 30 kDa, glycosylated; HEK)

Specification

Cat.No.

CSC-CTK0767

Description

Adiponectin, also referred to as Acrp30, AdipoQ and GBP-28, is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (adipokine). The protein belongs to the soluble defence collagen superfamily; it has a collagen-like domain structurally homologous with collagen VIII and X and complement factor C1q-like globular domain. Adiponectin forms homotrimers, which are the building blocks for higher order complexes found circulating in serum.

Species

Mouse

Product Overview

Mouse Adipoq expressed in HEK cells

Molecular Mass

30 kDa

Size

CAT# CSC-CTK0767-10 (10 μg); CAT# CSC-CTK0767-50 (50 μg)

Expression System

HEK cells

Purity

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

Activity

N/A

Endotoxin Level

N/A

Formulation

White, lyophilized (freeze-dried) powder. Sterile-f iltered and lyophilized from a 0.5 mg/ml protein solution in TBS buffer.

Reconstitution

Please centrifuge product briefly before opening vial. For reconstitution, add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. This solution can then be stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rMu Acrp30-HEK should be used immediately or stored long- term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles., cell culture and/or animal studies. Full-length adiponectin has been shown to activate AMP-activated protein kinase in hepatocytes (Yamauchi T. et al., 2002). It can also activate AMPK in HepG2 human hepatocytes at a concentration of 1.0 μg/ml. Full-length adiponectin purified from mammalian cells can acutely decrease blood glucose levels (Berg A.H. et al., 2001). Single subcutaneous injection of adiponectin at the dose of 30 mg/g body weight significantly decreased the blood glucose levels in C57 mice.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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