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ActoFactor™ Recombinant Human soluble Vascular Endothelial Growth Factor Receptor 1, Domain 1-3 (flt-1; Sf9 Insect)
- Specification
Cat.No.
CSC-CTK0406
Description
Recombinant human soluble Vascular Endothelial Growth Factor Receptor-1 D1-3 (sVEGFR-1 D1-3) is produced as a non-chimeric protein in a monomeric form. The soluble receptor protein contains only the first 3 extracellular domains, which contain all the information necessary for binding of VEGF. The receptor monomers have a mass of approximately 45 kDa containing 352 amino acid residues. Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor.
Species
Human
Product Overview
Human FLT1 expressed in Insect cells
Molecular Mass
45 kDa
Size
CAT# CSC-CTK0406-5 (5 μg); CAT# CSC-CTK0406-25 (25 μg)
Expression System
Insect cells
Purity
Greater than 90% as determined by SDS-PAGE analysis.
Activity
The activity of sVEGFR-1 was determined by its ability to inhibit the VEGF-A induced proliferation of HUVECs. The ED50 for this effect is typically between 12-30 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized (freeze-dried), sterile powder. Lyophilized with no additives.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein is soluble in water and most aqueous buffers. It should be reconstituted in sterile PBS to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C or -70°C. Reconstituted rHu sVEGFR-1 (D1-3) should be used immediately or stored long-term in undiluted working aliquots at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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