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ActoFactor™ Recombinant Human soluble TIE-2/Fc Chimera
- Specification
Cat.No.
CSC-CTK0596
Description
TIE-1 and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)- like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-2 cDNA encodes a 1124 amino acid (aa) precursor protein with an 18 aa putative signal peptide, a 727 aa extracellular domain and a 354 aa cytoplasmic domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind TIE-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. The recombinant mature TIE-2-Fc is a disulfide-linked homodimeric protein. Human TIE-2-Fc monomer has a calculated molecular mass of approximately 105 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 125 kDa protein in SDS-PAGE under reducing conditions. Recombinant human soluble TIE-2/Tek was fused with the Fc part of human IgG1. The recombinant mature sTIE-2/Fc is a disulfide-linked homodimeric protein. The sTIE-2/Fc monomers have a mass of approximately 125 kDa. The soluble receptor protein consists of the full extracellular domain (Met1-Val730).
Species
Human
Product Overview
Human TEK expressed in Insect cells
Molecular Mass
250 kDa
Size
CAT# CSC-CTK0596-20 (20 μg); CAT# CSC-CTK0596-100 (100 μg)
Expression System
Insect cells
Purity
Greater than 90% as determined by SDS-PAGE analysis.
Activity
Measured in a functional ELISA assay. When TIE-2/Fc is immobilized at 4 μg/ml (100 μl/well), it binds rh Angiopoietin-2 with a linear range of 2-100 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a steril-filtered protein solution in PBS.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile PBS or medium to a concentration of 0,1 - 1,0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu sTIE-2 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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