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ActoFactor™ Recombinant Human Vascular endothelial cell growth factor-EG
- Specification
Cat.No.
CSC-CTK0393
Description
Recombinant Human Endocrine Gland-derived Vascular Endothelial Cell Growth Factor (rHu EG- VEGF) is a secreted angiogenetic mitogen growth factor expressed by a variety of vascularized glands. The reported activities of human EG-VEGF include proliferation and migration in capillary endothelial cells derived from endocrine glands. RHu EG-VEGF is a 9.6 kDa protein consisting of 86 amino acid residues.
Species
Human
Product Overview
Human VEGFA expressed in E.coli
Molecular Mass
9.6 kDa
Size
CAT# CSC-CTK0393-20 (20 μg); CAT# CSC-CTK0393-100 (100 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Activity
The activity as determined by the dose-dependent proliferation of MIA PaCa-2 cells is typically 1- 4 μg/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered and lyophilized from a concentrated (1 mg/ml) solution with no additives.
Reconstitution
Please centrifuge product briefly before opening the vial. The lyophilized human EG-VEGF should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu EG-VEGF should be stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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