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ActoFactor™ Recombinant Human Tumor necrosis factor receptor superfamily, member 1A
- Specification
Cat.No.
CSC-CTK0347
Description
TNFR1 belongs to the TNFR superfamily of transmembrane proteins, and is expressed in most cell types. Binding of either TN-alpha or TNF-beta to TNFR1 initiates a signal transduction pathway that results in the activation of the transduction factor NFkB, whose target genes are involved in the regulation of inflammatory responses, and, in certain cells induce apoptosis. Soluble TNF Receptor I (sTNFR1) is capable of inhibiting TNF-alpha and TNF-beta activities by acting as a decoy receptor that serves as a sink for the TNF ligands. Recombinant human sTNFR1 is an 18.3 kDa protein (162 amino acid residues) comprising the cysteine rich ligand binding portion of the extracellular domain of the TNFR1 protein.
Species
Human
Product Overview
Human TNFRSF1A expressed in E.coli
Molecular Mass
18.3 kDa
Size
CAT# CSC-CTK0347-20 (20 μg); CAT# CSC-CTK0347-100 (100 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
Determined by the inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. The ED50 for this effect in the presence of 0.25 ng/ml of recombinant human TNF-alpha is 0.05 μg/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered, lyophilized.
Reconstitution
Please centrifuge product before opening vial. The lyophilized TNFR1 should be reconstituted in sterile 5 mM Tris (pH 8.0) to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu sTNFR1 should be used immediately or stored long-term in undiluted working aliquots at -20°C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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