ActoFactor™ Recombinant Human Tumor necrosis factor (ligand) superfamily, member 9

Specification

Cat.No.

CSC-CTK0490

Description

Human 4-1BB Ligand (4-1BBL) is a member of the emerging family of Ligands with structural homology to Tumor Necrosis Factor. Recombinant human 4-1BBL is a 19.5 kDa protein consisting of 185 amino acid residues.

Species

Human

Product Overview

Human TNFSF9 expressed in E.coli

Molecular Mass

19.5 kDa

Size

CAT# CSC-CTK0490-20 (20 μg); CAT# CSC-CTK0490-100 (100 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and HPLC analysis.

Activity

Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED50 for this effect is less than 10 ng/ml. The specific activity is 10^5 U/mg.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a 5 mM Tris/75 mM NaCl (pH 8.0) solution.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. It should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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