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ActoFactor™ Recombinant Human Trefoil factor 2
- Specification
Cat.No.
CSC-CTK0422
Description
The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are expressed in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF2 has been shown to inhibit gastrointestinal motility and gastric acid secretion. Recent data suggests a potential role for TFF2 in acute and chronic asthma (Nikolaidis, N.M. et al. Am. Journal Respir. Cell Mol. Biol. (2003) 4: 458-464). Recombinant human TFF2 is a 12.0 kDa polypeptide of 106 amino acid residues, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.
Species
Human
Product Overview
Human TFF2 expressed in E.coli
Molecular Mass
12.0 kDa
Size
CAT# CSC-CTK0422-20 (20 μg); CAT# CSC-CTK0422-100 (100 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by HPLC and SDS-PAGE analysis.
Activity
Determined by its ability to chemoattract human monocytes using a concentration range of 1.0- 10.0 μg/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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