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ActoFactor™ Recombinant Human Thrombopoietin (HEK)
- Specification
Cat.No.
CSC-CTK0428
Description
Recombinant Human Thrombopoietin (rHu TPO, Mpl-ligand, MGDF) is a glycoprotein hormone produced mainly by the liver and the kidney that regulates the production of platelets by the bone marrow. It stimulates the production and differentiation of megakaryocytes, the bone marrow cells that fragment into large numbers of platelets. Human recombinant Thrombopoietin produced in HEK cells is a glycosylated monomer with a molecular weight range of 80-85 kDa due to glycosylation. It has been purified by proprietary chromatographic techniques.
Species
Human
Product Overview
Human THPO expressed in HEK cells
Molecular Mass
N/A
Size
CAT# CSC-CTK0428-10 (10 μg); CAT# CSC-CTK0428-50 (50 μg)
Expression System
HEK cells
Purity
Greater than 90% as determined by SDS-PAGE analysis.
Activity
rHuTPO is fully biologically active when compared to a standard. The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is typically 0.5-5 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a concentrated protein solution (1 mg/ml) in 1xPBS.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu TPO should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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