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ActoFactor™ Recombinant Human Thrombopoietin
- Specification
Cat.No.
CSC-CTK0089
Description
Thrombopoietin (TPO) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. TPO stimulates the proliferation and maturation of
megakaryocytes, and has also been shown to promote the survival, self-renewal and expansion of hematopoietic stem cells and primitive multilineage progenitors.
Recombinant human TPO contains 332 amino acid residues and has a predicted molecular mass of approximately 35 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 47 kDa in SDS-PAGE.
Species
Human
Product Overview
Human THPO expressed in Sf21 insect cells
Sequence
A DNA sequence encoding the mature human TPO protein sequence (Ser 22 - Gly 353; Accession #P40225) was expressed in Sf21 insect cells.
Size
CAT# CSC-CTK0089-5 (5 μg); CAT# CSC-CTK0089-25 (25 μg)
Expression System
Sf21 insect cells
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The biological activity of recombinant human TPO is measured in a cell proliferation assay using MO7e cells. The ED50 for this effect is typically 0.3 - 3 ng/mL.
Endotoxin Level
Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
Formulation
Recombinant human TPO is lyophilized from a 0.2 μm filtered solution in 35% acetonitrile, 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg cytokine.
Reconstitution
Reconstitute human TPO at 50 μg/mL with sterile phosphate buffered saline (PBS) containing at least 0.1% human or bovine serum albumin.
Storage & Stability
Lyophilized human TPO is stable for up to twelve months from date of receipt at -20°C to -70°C. Reconstituted human TPO can be stored under sterile conditions
at 2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer) for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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