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ActoFactor™ Recombinant Human Neurotrophin 4
- Specification
Cat.No.
CSC-CTK0075
Description
Neurotrophin-4 (NT-4) is a member of the nerve growth factor (NGF) family of neurotrophins. It binds the TrkB receptor and acts as a survival factor for sensory neurons, although it has also been shown to sensitize cortical neurons to cell death (for a review, see 1). Together with other NGF family members NT-3 and BDNF, NT-4 has been described as a survival factor for human embryonic stem cells (hESC). Bioactive NT-3 is predicted to be a non-covalently linked homodimer. Mature recombinant NT-3 has a predicted molecular mass of approximately 13.6 kDa, with 119 amino acids following cleavage of the signal peptide and proprotein. The amino acid sequence of mature NT-3 is identical in human, mouse and rat.
Species
Human
Product Overview
Human NTF4 expressed in Sf21 insect cells
Sequence
The DNA sequence encoding the NT-3 prepropeptide3 was inserted in a baculovirus expression vector and expressed in Sf 21 insect cells.
Size
CAT# CSC-CTK0075-5 (5 μg); CAT# CSC-CTK0075-25 (25 μg)
Expression System
Sf21 insect cells
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The ED50 of NT-4, as measured by its ability to stimulate proliferation of the TrkB-transfected cell line, BaF-TrkB-BD, is typically 5 - 15 ng/mL.
Endotoxin Level
Endotoxin level is less than 1.0 EU/μg of NT-4, as determined by the LAL method.
Formulation
Lyophilized from a sterile-filtered solution in 30% acetonitrile plus 0.1% TFA containing 50 μg of bovine serum albumin per 1 μg of NT-4.
Reconstitution
It is recommended that a stock solution at a concentration of no less than 10 μg/mL be prepared in sterile phosphate buffered saline containing at least 0.1% human or bovine serum albumin.
Storage & Stability
The lyophilized sample is stable for up to 1 year at -20°C to -70°C. Reconstituted NT-4 can be stored under sterile conditions at 2 - 8°C for 1 month or at -20°C to -70°C for 3 months in a manual defrost freezer without detectable loss of acti vity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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