ActoFactor™ Recombinant Human Neurotrophin 3

Specification

Cat.No.

CSC-CTK0487

Description

Recombinant Human Neurotrophin-3 (rHu NT-3) is a potent neurotrophic factor, which supports the growth and survival of nerve and glial cells. Human NT-3 produced in E. coli is a non- glycosylated and non-covalently linked homodimer containing 2 x 119 amino acids chains and having a molecular mass of 27.2 kDa. Recombinant human NT-3 has been purified using proprietary chromatographic techniques.

Species

Human

Product Overview

Human NTF3 expressed in E.coli

Molecular Mass

27.2 kDa

Size

CAT# CSC-CTK0487-10 (10 μg); CAT# CSC-CTK0487-50 (50 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and RP-HPLC.

Activity

The ED50 as determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cultures was found between 20-50 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

The sterile-filtered protein solution in water (1 mg/ml) was lyophilized with no additives.

Reconstitution

Please centrifuge product briefly before opening the vial. The lyophilized powder should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu NT-3 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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