ActoFactor™ Recombinant Human Neuropilin 1 (E. coli)

Specification

Cat.No.

CSC-CTK0398

Description

Vascular Endothelial Cell Growth Factor (VEGF) is a secreted homodimeric protein expressed by a variety of vascularized tissues. The reported activities of human VEGF include stimulation of endothelial cell growth, angiogenesis, and vascular permeability. Human VEGF is a 38.2 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains.

Species

Human

Product Overview

Human NRP1 expressed in E.coli

Molecular Mass

38.2 kDa

Size

CAT# CSC-CTK0398-10 (10 μg); CAT# CSC-CTK0398-50 (50 μg)

Expression System

E.coli

Purity

Greater than 95% as determined by SDS-PAGE analysis.

Activity

The ED50 for stimulation of cell proliferation in human umbilical vein endothelial cells by VEGF- 165 has been determined to be in the range of 1-5 ng/ml.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a protein solution in 50 mM acetic acid with no additives.

Reconstitution

Please centrifuge product briefly before opening the vial. The lyophilized human VEGF should be reconstituted in sterile 50 mM acetic acid to a concentration not lower than 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu VEGF should be stored long-term in undiluted working aliquots at –20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

Q & A
Customer Review

Ask a Question

Write your own review

ONLINE INQUIRY