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ActoFactor™ Recombinant Human Nerve growth factor
- Specification
Cat.No.
CSC-CTK0480
Description
Pro-NGF is the pro-form of the neurotrophin nerve growth factor. Like the mature protein Pro- NGF is characterized by the cysteine knot motif consisting of three cysteine bridges. The protein predominantly exists as a non-covalently linked homodimer. Recombinant human Pro-NGF produced in E. coli is a non-glycosylated polypeptide chain of 222 amino acids and a molecular mass of 49,738 Dalton.
Species
Human
Product Overview
Human NGF expressed in E.coli
Molecular Mass
49.7 kDa
Size
CAT# CSC-CTK0480-10 (10 μg); CAT# CSC-CTK0480-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
The activity of the protein can by measured by its stimulating effect on the proliferation of TF1 cells (Chevalier et al. 1994, Blood 83, 1479-85). EC50 = 130 ± 30 pM (TF1 cell assay).
Endotoxin Level
Less than 1 EU/μg.
Formulation
The protein solution (1.2 mg/ml; 10 μg/9 μl) contai ns 50 mM sodium phosphate buffer (pH 7.2) and 150 mM sodium chloride.
Reconstitution
Please centrifuge product briefly before opening vial. This protein solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The protein solution, though stable at 15°C for up to 3 weeks, is best stored desiccated at 4°C. It should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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