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ActoFactor™ Recombinant Human Nanog homeobox
- Specification
Cat.No.
CSC-CTK0379
Description
Nanog is a regulatory protein that is associated with undifferentiated pluripotent cells. The expression of Nanog, which is suppressed in all adult tissues, is restricted to embryonic stem cells and to certain pluripotent cancer cells. Decreased expression of Nanog is strongly correlated with cell differentiation. Nanog, most likely, acts as an intracellular regulator, which maintains pluripotency and self renewal via a STAT3 independent pathway. Recombinant human Nanog is a 34.5 kDa protein, which is synthesized as a 304 amino acid polypeptide lacking a signal sequence for secretion.
Species
Human
Product Overview
Human NANOG expressed in E.coli
Molecular Mass
34.5 kDa
Size
CAT# CSC-CTK0379-20 (20 μg); CAT# CSC-CTK0379-100 (100 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
N/A
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu Nanog should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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