ActoFactor™ Recombinant Human Matrix metallopeptidase 3 (HEK)

Specification

Cat.No.

CSC-CTK0370

Description

MMP-3 enzyme is also known as Stromelysin-1 or as Transin-1 which hydrolyzes natural collagen at physiological pH and temperature. MMP-3 hydrolyzes components of the extracellular matrix like proteoglycan, laminin, fibronectin, gelatin and collagen types III, IV and IX. It also activates pro-MMP-9 and pro-MMP-8 and superactivates plasmin activated MMP-1. MMP-3 is secreted as a latent proenzyme and is activated by a variety of proteinases, e.g. plasmin, trypsin, chymotrypsin, cathepsin G or human neutrophil elastase. MMP-3 was found to be capable of activating the precursor of IL-1 beta.|Human recombinant MMP-3 produced in HEK293 cells is a proform of the Human MMP3 [Tyr18-Cys477 (Lys45Glu)] and fused with a ployhistide tag at the C-terminus. It has an MW of 52 kDa and has been purified by using proprietary chromatographic techniques.

Species

Human

Product Overview

Human MMP3 expressed in HEK cells

Molecular Mass

52 kDa

Size

CAT# CSC-CTK0370-10 (10 μg); CAT# CSC-CTK0370-50 (50 μg)

Expression System

HEK cells

Purity

Greater than 95% as determined by SDS-PAGE analysis.

Activity

The activity was measured by its ability to cleave the fluorogenic peptide substrate, Mca- RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >150 pmoles/min/μg.

Endotoxin Level

N/A

Formulation

Sterile-filtered (0.2 μm), colorless solution in Tris, NaCl and Brij35.

Reconstitution

Please centrifuge product briefly before opening vial. The protein solution can be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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