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ActoFactor™ Recombinant Human Matrix Metalloproteinase 9
- Specification
Cat.No.
CSC-CTK0373
Description
Matrix metalloproteinases are a family of zinc and calcium-dependent endopeptidases that break down extracellular matrix proteins. The MMP-9 is secreted as a 92 kDa zymogen. Cleavage of ProMMP-9 results in the active enzyme, having a molecular weight of approximately 82 kDa. MMP-9 is composed of the following domains: a gelatin-binding domain consisting of three fibronectin type II units, a catalytic domain containing the zinc-binding site, a proline-rich type V collagen-homologous domain and a hemopexin-like domain. MMP-9 is produced by the several cell types: monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts and endothelial cells. MMP-9 is involved in inflammatory responses, tissue remodeling, wound healing, tumor growth and metastasis. MMP-9 may also play an important part in local proteolysis of the extracellular matrix and in leukocyte migration, as well as in bone osteoclastic resorption. MMP-9 cleaves type IV and type V collagens into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. MMP-9 can also degrade fibronectin but not laminin or Pz-peptide. MMP-9 defects may be a cause of susceptibility to intervertebral disc disease (IDD), also known as lumbar disk herniation (LDH). Human recombinant MMP-9 produced in E. coli is a single, non-glycosylated polypeptide chain that contains a 338 amino acids fragment (113-450; corresponding to the catalytic domain of the protein) with a total molecular mass of 42.03 kDa and that is fused with a 4.5 kDa amino- terminal hexahistidine tag.
Species
Human
Product Overview
Human MMP9 expressed in E.coli
Molecular Mass
46 kDa
Size
CAT# CSC-CTK0373-10 (10 μg); CAT# CSC-CTK0373-50 (50 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
N/A
Endotoxin Level
N/A
Formulation
MMP-9 protein is supplied in 20 mM Tris-HCl (pH 8.0) and 50% glycerol.
Reconstitution
Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. Can be used as standard in Western blots, etc.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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