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ActoFactor™ Recombinant Human Interleukin 7
- Specification
Cat.No.
CSC-CTK0088
Description
nterleukin 7 (IL-7) is a potent lymphoid cell growth factor which affects Pre-B, Pro-B and early T cells. It will also affect mature T cells in combination with other factors such as IL-2. Human IL-7 is a 17 kDa protein containing 153 amino acid residues.
Species
Human
Product Overview
Human IL7 expressed in E.coli
Sequence
A DNA sequence encoding human IL-7 was expressed in E. coli.
Size
CAT# CSC-CTK0088-5 (5 μg); CAT# CSC-CTK0088-25 (25 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The ED50 measured as T cell growth factor activity in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes is typically 0.2-0.5 ng/ml, corresponding to a specific activity of 2 x 10^6 units/mg.
Endotoxin Level
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Formulation
Lyophilized from a sterile-filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
Reconstitution
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 mg/ml of the cytokine.
Storage & Stability
The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted IL-7 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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