ActoFactor™ Recombinant Human Interleukin 2

Specification

Cat.No.

CSC-CTK0004

Description

Interleukin 2 (IL-2) is a potent lymphoid cell growth factor which exerts its biological activity primarily on T cells. The recombinant human IL-2 contains 134 amino acid residues and has a predicted molecular mass of approximately 15 kDa.

Species

Human

Product Overview

Human IL2 expressed in E.coli

Sequence

A DNA sequence encoding human IL-2 was expressed in E. coli.

Size

CAT# CSC-CTK0004-10 (10 μg); CAT# CSC-CTK0004-50 (50 μg)

Expression System

E.coli

Purity

Greater than 97% as determined by SDS-PAGE and visualized by silver stain

Activity

Measured in a cell proliferation assay using the IL-2 dependent murine cytotoxic T cell line, CTLL-2, the ED50 is typically 0.05 - 0.25 ng/mL.

Endotoxin Level

Endotoxin level is less than 1 EU per 1 ug of the cytokine, as determined by the LAL method.

Formulation

Recombinant human IL-2 is lyophilized from a sterile 0.2 μm filtered solution in Acetonitrile and TFA containing bovine serum albumin.

Reconstitution

Reconstitute human IL-2 with sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin to prepare a stock solution of 10 ?g/mL of the cytokine.

Storage & Stability

Lyophilized human IL-2 is stable for up to twelve months from date of receipt at -20°C to -70°C. Reconstituted human IL-2 can be stored under sterile conditions at 2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer) for three months without detectable loss of activity. Avoid repeated freezing and thawing.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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