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ActoFactor™ Recombinant Human Insulin-like growth factor 2 (somatomedin A)
- Specification
Cat.No.
CSC-CTK0057
Description
Insulin-like growth factor II (IGF-2) and IGF-I are family members of insulin-like growth factors that are structurally homologous to proinsulin, and share approximately 70% sequence identity. IGF-2 is expressed in many tissues and autocrine, paracrine and endocrine functions have been reported. Mature IGF-2 is highly conserved and has cross-species reactivity. Two specific cell surface receptors, type I IGF and type II IGF receptors, that bind IGF-1 and IGF-2 have been identified. Recombinant human IGF-2 contains 67 amino acids and has a predicted molecular mass of approximately 7.5 kDa.
Species
Human
Product Overview
Human IGF2 expressed in E.coli
Sequence
A DNA sequence encoding the mature IGF-2 protein was expressed in E. coli.
Size
CAT# CSC-CTK0057-50 (50 μg); CAT# CSC-CTK0057-250 (250 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
Activity was determined in a serum-free cell proliferation assay using the human cell line MCF-7 and the ED50 for this effect was typically 5.0 - 10.0 ng/mL.
Endotoxin Level
Endotoxin level less than 0.1 ng per 1 mg of the cytokine as determined by the LAL method.
Formulation
Lyophilized from a 0.2 μm filtered solution in 0.1 M acetic acid.
Reconstitution
It is recommended that PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10 mg/mL of the cytokine.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted hIGF-2 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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