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ActoFactor™ Recombinant Human Hepatocyte Growth Factor, A Chain
- Specification
Cat.No.
CSC-CTK0415
Description
Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3 and GM-CSF to stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis. HGF is secreted as a single inactive polypeptide which is cleaved by serine proteases into a 69 kDa Alpha chain and 34 kDa Beta chain. A disulfide bond linking the alpha and beta chains produces the active, heterodimeric molecule. Human recombinant HGF-A produced in E. coli is a single, non-glycosylated, polypeptide chain containing a 463 amino acids fragment (32-494) and having a molecular weight of 69 kDa. It is fused with a 4.5 kDa amino-terminal hexahistidine tag.
Species
Human
Product Overview
Human HGF expressed in E.coli
Molecular Mass
N/A
Size
CAT# CSC-CTK0415-10 (10 μg); CAT# CSC-CTK0415-50 (50 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
N/A
Endotoxin Level
N/A
Formulation
Sterile-filtered, clear protein solution (0.105 mg/m l) in 22 mM sodium acetate (pH 4.8), 1 mM EDTA and 50% glycerol.
Reconstitution
Please centrifuge product briefly before opening vial. The dissolved protein can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
Store at 4°C if entire vial will be used within 4 weeks. Store frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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