ActoFactor™ Recombinant Human Growth Differentiation Factor 5 (BMP-14)

Specification

Cat.No.

CSC-CTK0540

Description

Recombinant human GDF-5 produced in E. coli is a homodimeric, non-glycosylated protein containing 2 x 117 amino acid chains and having a total molecular mass of 26.8 Dalton. To enable bacterial expression of rhGDF-5 we removed the N-terminal sequence Ala-Pro-Leu-Thr and added Lys.

Species

Human

Product Overview

Human GDF5 expressed in E.coli

Molecular Mass

26.8 kDa

Size

CAT# CSC-CTK0540-50 (50 μg); CAT# CSC-CTK0540-250 (250 μg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and RP-HPLC analysis.

Activity

Human GDF-5 is fully biologically active when compared to a standard. Induction of alkaline phosphatase activity in ATDC5 cells: EC50 = 40 Nm.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized without any additives.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile 20 mM AcOH to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu GDF-5 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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