Featured Products
- HCC-78
- HDLM-2
- DOHH-2
- L-540
- MX-1
- NALM-6
- NB-4
- CAL-51
- SNB-19
- KYSE-520
- MKN-45
- BA/F3
- MS-5
- HCEC-B4G12
- NK-92
- PA-TU-8988S
- MONO-MAC-1
- PA-TU-8902
- Human Microglia
- Human Hepatic Stellate Cells
- Human Skeletal Muscle Cells (DMD)
- Human Schwann Cells
- Human Oral Keratinocytes (HOK)
- Human Cardiomyocytes
- Human Small Intestinal Epithelial Cells
- Human Colonic Epithelial Cells
- Human Intestinal Fibroblasts
- Primary Human Large Intestine Microvascular Endothelial Cells
- Human Small Intestinal Microvascular Endothelial Cells
- Human Retinal Pigment Epithelial Cells
- Human Hepatocytes
- Cynomolgus Monkey Lung Microvascular Endothelial Cells
- Cynomolgus Monkey Vein Endothelial Cells
- C57BL/6 Mouse Primary Mammary Epithelial Cells
- C57BL/6 Mouse Vein Endothelial Cells
- Rat Primary Kidney Epithelial Cells
- Rat Gingival Epithelial Cells
- Rabbit Lung Endothelial Cells
Our Promise to You
Guaranteed product quality, expert customer support
ActoFactor™ Recombinant Human Glial Derived Neurotropic Factor (E. coli)
- Specification
Cat.No.
CSC-CTK0484
Description
Human Glial Derived Neurotrophic Factor (rHu GDNF) is a 30.4 kDa protein that specifically promotes dopamine uptake and survival of midbrain neurons. rHu GDNF is a disulfide-linked homodimeric protein consisting of two identical 135 amino acid chains.
Species
Human
Product Overview
Human BDNF expressed in E.coli
Molecular Mass
30.4 kDa
Size
CAT# CSC-CTK0484-10 (10 μg); CAT# CSC-CTK0484-50 (50 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
The ED50 as determined by the dose-dependent dopamine uptake by rat ventral mesencephalic cultures was found to be in the range of 5-10 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
The sterile filtered solution was lyophilized from 10 mM NaCitrate (pH=4), 150 mM NaCl.
Reconstitution
Please centrifuge product before opening the vial. The lyophilized protein is soluble in water and most aqueous buffers. RHu GDNF should be reconstituted to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into water or other buffered solutions and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature, is best stored desiccated at -20°C. Reconstituted rHu GDNF should be stored in working aliquots at -20°C. Please avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Ask a Question
Write your own review
ONLINE INQUIRY
