ActoFactor™ Recombinant Human Fibroblast growth factor 8 (androgen-induced) (E. coli)

Specification

Cat.No.

CSC-CTK0145

Description

Recombinant Human Fibroblast Growth Factor-8 (rHu FGF-8), also known as Androgen-induced growth factor (AIGF), is a heparin binding growth factor, which stimulates the proliferation and activation of cells that express FGF receptors. Creative Bioarrays human FGF-8 is a 22.4 kDa protein containing 193 amino acid residues.

Species

Human

Product Overview

Human FGF8 expressed in E.coli

Molecular Mass

22.4 kDa

Size

CAT# CSC-CTK0145-25 (25 μg); CAT# CSC-CTK0145-125 (125 μg)

Expression System

E.coli

Purity

Greater than 95% as determined by SDS-PAGE and HPLC analysis.

Activity

The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 0.5 ng/ml, corresponding to a specific activity of Greater than 2×10^6 units/mg.

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyopholized from 10mM Tris pH 8.0 + 75mM NaCl.

Reconstitution

Please centrifuge product before opening vial. The lyophilized FGF-8 should be reconstituted in 5mM Tris, pH 8.0 to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature, is best stored dessicated at -20°C. Reconstituted rHu FGF-8 should be used immediately or stored in working aliquots at -20°C. Please avoid repeated freeze / thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

Q & A
Customer Review

Ask a Question

Write your own review

ONLINE INQUIRY