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ActoFactor™ Recombinant Human Fibroblast growth factor 6
- Specification
Cat.No.
CSC-CTK0144
Description
FGF-6 is a secreted heparin binding growth factor that is a member of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF-6 is expressed in leukemia cell lines with platelet megakaryocytic differentiation potential. It signals through FGFR 1c, 2c, and 4. Recombinant human FGF-6 is an 18.7 kDa protein consisting of 168 amino acid residues.
Species
Human
Product Overview
Human FGF6 expressed in E.coli
Molecular Mass
18.7 kDa
Size
CAT# CSC-CTK0144-25 (25 μg); CAT# CSC-CTK0144-125 (125 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
The ED50, as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors, is 2 x 10^6 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Sterile, lyophilized (freeze-dried) powder. Lyophilzed from 10 mM Sodium Phospate (pH 7.2) and 50 mM NaCl.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized FGF-6 should be reconstituted in sterile 5 mM sodium phosphate (pH 7.2) to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into water or other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu FGF-6 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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