ActoFactor™ Recombinant Human Epidermal growth factor (E. coli)

Specification

Cat.No.

CSC-CTK0126

Description

Recombinant Human Epidermal Growth Factor (rHu EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal and epithelial cells. rHu EGF produced in E. coli is a single, non-glycosylated polypeptide chain of 53 amino acids and a molecular mass of 6,222 Dalton.

Species

Human

Product Overview

Human EGF expressed in E.coli

Molecular Mass

6.2 kDa

Size

CAT# CSC-CTK0126-500 (500 μg); CAT# CSC-CTK0126-2500 (2.5 mg)

Expression System

E.coli

Purity

Greater than 98% as determined by SDS-PAGE and SEC-HPLC analysis.

Activity

Greater than 1.0×10^7 IU/mg

Endotoxin Level

Less than 1 EU/μg.

Formulation

Lyophilized from a sterile-filtered, concentrated protein solution in PBS (pH 7.4) with no additives.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized rHu EGF should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, should be stored desiccated at -20°C. Reconstituted rHu EGF should be used immediately or stored in undiluted working aliquots at -20°C. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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