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ActoFactor™ Recombinant Human Defensin, beta 4A
- Specification
Cat.No.
CSC-CTK0432
Description
Defensins are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. To date, four human beta-defensins have been identified: BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on leukocytes and at epithelial surfaces and are 3-5 kDa peptides consisting of 33-47 amino acid residues. Creative Bioarrays recombinant human beta-defensin-2 (rHu BD-2) is a 4.3 kDa protein containing 41 amino acid residues.
Species
Human
Product Overview
Human DEFB4A expressed in E.coli
Molecular Mass
4.3 kDa
Size
CAT# CSC-CTK0432-20 (20 μg); CAT# CSC-CTK0432-100 (100 μg)
Expression System
E.coli
Purity
Greater than 98% as determined by SDS-PAGE and HPLC analysis.
Activity
The biological activity was determined by its ability to chemoattract immature dendritic cells using a concentration of 10-100 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
The sterile filtered solution was lyophilized with no additives.
Reconstitution
Please centrifuge product before opening the vial. The lyophilized protein should be reconstituted in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized BD-2, though stable at room temperature, is best stored desiccated at -20°C. Reconstituted BD-2 should be stored in working aliquots at -20°C. Please avoid freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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