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ActoFactor™ Recombinant Human Defensin, beta 1 (36 aa variant)
- Specification
Cat.No.
CSC-CTK0433
Description
Defensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. Beta-defensins are expressed on some leukocytes and at epithelial surfaces. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The Beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1 (36 a.a.), a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Beta-Defensins are 3-5 kDa peptides ranging in size from 33-47 amino acid residues. Recombinant Human BD-1 is a 3.9 kDa protein containing 36 amino acid residues.
Species
Human
Product Overview
Human DEFB1 expressed in E.coli
Molecular Mass
3.9 kDa
Size
CAT# CSC-CTK0433-20 (20 μg); CAT# CSC-CTK0433-100 (100 μg)
Expression System
E.coli
Purity
Greater than 95% as determined by SDS-PAGE and HPLC analysis.
Activity
Determined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 100.0-1000.0 ng/ml.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Lyophilized from a sterile-filtered solution in 10 mM sodium citrate.
Reconstitution
Please centrifuge product briefly before opening the vial. Reconstitute in sterile 10 mM sodium citrate to a concentration of 0.1-1.0 mg/ml. This solution can be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted BD-1 should be used immediately or stored in undiluted working aliquots at -20°C. Avoid repeated freeze / thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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