ActoFactor™ Recombinant Human Colony stimulating factor 3 (granulocyte-macrophage)

Specification

Cat.No.

CSC-CTK0152

Description

GM-CSF is a cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. It is involved in differentiation of dendritic cells and is a key factor in differentiation pathways originating from stem cells. GM-CSF is produced by several cell types as monocytes, fibroblasts, endothelial cells and T- Lymphocytes in response to a number of inflammatory mediators present in the hematopoietic environment and peripheral site of inflammation. Human GM-CSF is an important therapeutic cytokine used in the treatment of myeloid leukemia, neutropenia and aplastic anemia and it could become interesting in the treatment following bone marrow transplantation. It performs biological activity by binding to a specific receptor complex which is composed of a cytokine-specific alpha chain and B chain shared with the receptors for Interleukin-3 and Interleukin-5. GM-CSF has been identified to mediate the activation of Jak-Stat and MAPK pathways. Recombinant human GM-CSF is a glycosylated polypeptide chain containing 127 amino acids (18-144 aa CSF2_HUMAN P04141 ), fused to 10XHis tag at the N-terminus. It migrates as a broad band between 15 and 25 kDa due to post-translation modification, in particular glycosylation. Human recombinant GM-CSF expressed in Nicotiana benthamiana is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product does not contain any animal–derived components or impurities.

Species

Human

Product Overview

Human CSF3 expressed in Nicotiana benthamiana

Molecular Mass

N/A

Size

CAT# CSC-CTK0152-10 (10 μg); CAT# CSC-CTK0152-50 (50 μg)

Expression System

Nicotiana benthamiana

Purity

Greater than 97% as determined by SDS-PAGE analysis.

Activity

The activity of recombinant human GM-CSF is determined by the dose-dependent induction of human TF-1 proliferation cell. The measured ED50 is ≤ 0.05 ng/ml, corresponding to a specific activity of Greater than 2.0 x 10^7 units/mg.

Endotoxin Level

Less than 0.04 EU/μg.

Formulation

Lyophilized from a concentrated, sterile protein solution in 10 mM PBS buffer (pH 7.6) and 0.2 M NaCl.

Reconstitution

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 25-50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. Optimal concentration should be determined for specific application and cell lines.

Storage & Stability

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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