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ActoFactor™ Recombinant Human Colony stimulating factor 3 (granulocyte) (CHO)
- Specification
Cat.No.
CSC-CTK0160
Description
Recombinant Human Granulocyte Stimulating Factor (rHu G-CSF) is a potent stimulator of bone marrow cells, especially those of neutrophil lineage. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17. Human G-CSF is an 20 kDa glycosylated protein containing 174 amino acid residues.
Species
Human
Product Overview
Human CSF3 expressed in CHO cells
Molecular Mass
20 kDa
Size
CAT# CSC-CTK0160-10 (10 μg); CAT# CSC-CTK0160-50 (50 μg)
Expression System
CHO cells
Purity
Greater than 97% as determined by SDS-PAGE, FPLC and RP-HPLC analysis.
Activity
Recombinant G-CSF is fully biologically active when compared to a standard. The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake), is less than 0.07 ng/ml, corresponding to a specific activity of 1.27 x 10^8 IU/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
Human G-CSF was lyophilized from a concentrated (1 mg/ml) Phosphate-Buffered Saline, pH 7.4.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu G-CSF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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