ActoFactor™ Recombinant Human Colony stimulating factor 1 (macrophage)

Specification

Cat.No.

CSC-CTK0026

Description

Macrophage-Colony stimulating factor (M-CSF or CSF-1) can be produced by a number of cells including fibroblasts, activated macrophages, secretory epithelial cells of the endometrium, bone marrow stromal cells, and LPS- or cytokine-activated endothelial cells. M-CSF not only stimulates the formation of macrophage colonies from bone marrow hematopoietic progenitor cells, but also induces proliferation of isolated macrophages, enhances macrophage antibody-dependent cell-mediated cytotoxicity, induces priming and enhancing of macrophages ability to kill tumor cells and microorganisms, regulates release of cytokines and other inflammatory modulators from macrophages, stimulates pinocytosis and osteoclast differentiation. M-CSF is synthesized as a membrane-bound propeptide. Subsequent to proteolytic processing, M-CSF can occur either as a secreted, soluble form or as a membrane-anchored form, both of which are biologically active. Natural M-CSFs are glycosylated, disulfide-linked, homodimeric proteins with molecular weights ranging from 40-70 kDa. Several C-terminal variants of the soluble form of M-CSF have been found. The N-terminal 150 amino acid residues of the mature M-CSF, a region necessary and sufficient for interaction with the M-CSF receptor, is highly conserved (80% homology) between the human and murine proteins. Human M-CSF is active in the murine system, but murine M-CSF is species-specific. M-CSF is a glycoprotein, but the carbohydrate moiety is not necessary for biological activity.

Species

Human

Product Overview

Human CSF1 expressed in E.coli

Sequence

A DNA sequence encoding the N-terminus 158 amino acid residues of the extracellular domain of the mature native human M-CSF protein sequence was expressed in E. coli.

Size

CAT# CSC-CTK0026-5 (5 μg); CAT# CSC-CTK0026-25 (25 μg)

Expression System

E.coli

Purity

Greater than 97% as determined by SDS-PAGE and visualized by silver stain

Activity

Activity was determined by cell proliferation assay in M-NFS-60 cells and the ED50 using this assay was typically 0.5 – 1.5 ng/ml.

Endotoxin Level

Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.

Formulation

Lyophilized from a sterile-filtered solution of phosphate-buffered saline containing 50 mg of bovine serum albumin per 1 mg of cytokine.

Reconstitution

It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 mg/ml of the cytokine.

Storage & Stability

The lyophilized sampleis stable for greater than six months at -20 °C to -70°C. Reconstituted M-CSF can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to-70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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